Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays

Abstract Excess glutamate in the central nervous system may be a major cause of neurodegenerative diseases with gradual loss and dysfunction neurons. Primary or secondary metabolites from medicinal plants algae show potential for treatment glutamate-induced excitotoxicity. Three plant extracts were evaluated impact on excitotoxicity-induced primary cultures retinal ganglion cells (RGC). These treated separately seven groups: control; Plicosepalus. curviflorus treated; Saussurea lappa Cladophora glomerate treated. Cells independently 5, 10, 50, 100 µg/ml alga material , respectively, 2 h. Glutamate-treated (48 h µM glutamate); P. /glutamate; S. C. glomerata /glutamate [pretreatment extract (50 µg/ml) before 48 h]. Comet MTT assays used to assess cell damage viability. The number viable fell significantly after exposure. Exposure caused no notable effect All tested showed protective against RGC death. Use these neurological conditions related excitotoxicity oxidative stress might prove beneficial.